Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:1000g Yeast extract 10 Glucose 100

Yeast extract 10 Glucose 100 Potassium Acetate 20 Agar 20 Suspend 150 gram powdered medium in 1 litre distilled water

MES was developed as one of Good's buffers in the 1960s

20 pH (100 g/l) 11

2 at 25°C Suspend 50 gram powdered medium in 1 litre distilled water Store dry at room temperature

0 Beef Extract 5

Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Size:1000g Yeast extract 10 Glucose 100Calcium chloride, CaCl2 is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of calcium or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium Nitrate. Suspend 1. 8 gram powdered medium in 1 litre distilled water Store dry at room